![]() This would allow use of ICSI by owners or veterinarians who do not currently have access to it. Development of systems for transporting oocytes would expand access to the technique oocytes could be recovered by the referring veterinarian and shipped to the ICSI facility for embryo production. Unfortunately, ICSI requires expensive equipment and is technically demanding, limiting its availability to a few locations. In vitro embryo production via intracytoplasmic sperm injection (ICSI) is a useful tool to produce foals from mares unable to produce their own embryos, from stallions with limited sperm numbers or sperm quality and even from mares post mortem 1- 4. This new, practical information supporting transport of both immature and DSF oocytes for ICSI may allow wider use of this procedure. ![]() Better blastocyst rates per oocyte are obtained from DSF oocytes however, these require maintenance at 37☌ and as they are already maturing at the time of collection, are more sensitive to delays. ConclusionsĪ commercially available medium can be used for shipping immature oocytes at room temperature with good resulting blastocyst rates. Immature oocytes held for one or 2 nights in modified M199 medium, or for one night in commercial embryo holding solution, in air at room temperature, yielded 35–37% blastocyst development per injected oocyte. Dominant stimulated follicle oocytes held similarly in a warm passive device yielded poor blastocyst development (10%). Dominant stimulated follicle oocytes held in sealed tubes in pre-equilibrated control maturation medium maintained at 37☌ yielded blastocyst development equal to that obtained for control incubated oocytes (70%). Resultsīlastocysts were produced in all groups. The rate of blastocyst production was compared among treatments. To mimic transport, DSF oocytes were incubated overnight under differing conditions before ICSI immature oocytes were placed in varying conditions overnight before in vitro maturation, followed by ICSI. Oocytes were recovered by transvaginal ultrasound-guided follicular aspiration either from dominant follicles 24 h after deslorelin administration (dominant stimulated follicle ), or from subordinate (immature) follicles at the same time. This study was conducted to evaluate protocols that could be used to transport immature and maturing oocytes for ICSI. Methods to transport oocytes to ICSI laboratories would allow collection of oocytes by the referring veterinarian and enable greater application of this technique. Intracytoplasmic sperm injection (ICSI) is used to produce foals from otherwise infertile mares and from stallions with limited sperm stores, but requires expensive equipment and is technically demanding.
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